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rabbit anti trpm5  (Proteintech)


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    Structured Review

    Proteintech rabbit anti trpm5
    Rabbit Anti Trpm5, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 64 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+tff2/TFF2+Antibody/us12590288-3395-100-99
    Average 93 stars, based on 64 article reviews
    rabbit anti trpm5 - by Bioz Stars, 2026-09
    93/100 stars

    Images

    Related Articles

    Staining:

    Article Title: A novel telomere biology disease‐associated gastritis identified through a whole exome sequencing‐driven approach
    Article Snippet: After antigen retrieval treatment (epitope retrieval solution II AR9640, Leica Biosystems) for 20 minutes, anti-CCKBR antibody (1:200) was applied on tissue sections for 60 minutes incubation and the antigen-antibody binding was detected with (ImmPress anti-goat polymer HRP, MP7405) and DAB (Bond Polymer Refine Detection). .. The tissue sections were counterstained with hematoxylin and covered with cover glasses. (CCKBR: brown cytoplasmic stain, Ki67: red nuclear staining) f. Protocol for anti-TFF2 (ProteinTech Group, catalog# 13681-1-AP, rabbit polyclonal, 400 μg/ml) and Ki67 (RM-9106-AS1, ThermoFisher, rabbit monoclonal, clone SP6) on human GI paraffin tissues: The slide was stained using Leica Bond RX automated stainer. .. After deparaffinization and rehydration, tissue section was heat treated for 20 minutes with antigen retrieval solution (Leica Biosystems, AR9961).

    Article Title: A novel telomere biology disease‐associated gastritis identified through a whole exome sequencing‐driven approach
    Article Snippet: Anti-Pepsinogen 1 antibody (1:400) was applied on tissue sections for one-hour incubation at room temperature and the antigen-antibody binding was detected with Bond Polymer Refine Detection (Leica Biosystems, DS9800). .. Tissue sections were briefly immersed in hematoxylin for counterstaining and were covered with cover glasses. c. Protocol for anti-TFF2 (ProteinTech Group, catalog# 13681-1-AP, rabbit polyclonal, 400 μg/ml) on human FFPE GI tissues: The slide was stained using Leica Bond RX automated stainer. .. After deparaffinization and rehydration, tissue section was heat treated for 20 minutes with antigen retrieval solution (Leica Biosystems, AR9961).

    Incubation:

    Article Title: Proteomic Landscape of Small Extracellular Vesicles Derived From Gastric Juice and Identified TFF2 as a Specific Biomarker
    Article Snippet: For CD9, human samples were incubated with anti-CD9 (20,597-1-AP, Proteintech), and mouse samples with anti-CD9 (ab307085, Abcam). .. For TFF2, murine samples were treated with anti-TFF2 (13,681-1-AP, Proteintech), while human samples were incubated with anti-TFF2 (ab267474, Abcam). ..

    Article Title: Role of Innate Oral Immunity and the Salivary Fluid in Inflammatory Bowel Disease
    Article Snippet: MIF or TFF2 protein level in saliva collected from WT mice before and after DSS treatment was measured by commercial sandwich enzyme-linked immunosorbent assay (ELISA) kits (MyBioSource, MIF Cat no. MBS164186, TFF2 MBS763147) according to the kit protocol. .. The depletion of MIF or TFF2 protein from the saliva fluid was by incubation of the fluid for overnight at 4°C with anti-MIF (Cell Signaling, Cat no.88186S) or anti-TFF2 (ProteinTech, Cat no. 13681-1-AP) neutralizing antibodies at a dilution of 5 μg/mL for both peptides. ..

    Article Title: Proteomic Landscape of Small Extracellular Vesicles Derived From Gastric Juice and Identified TFF2 as a Specific Biomarker
    Article Snippet: For CD9, human samples were incubated with anti-CD9 (20,597-1-AP, Proteintech), and mouse samples with anti-CD9 (ab307085, Abcam). .. For TFF2, murine samples were treated with anti-TFF2 (13,681-1-AP, Proteintech), while human samples were incubated with anti-TFF2 (ab267474, Abcam). ..

    Article Title: The expression of trefoil factor family member 2 in increased at an acidic pH.
    Article Snippet: The cells were permea‐ bilized with 0.2% Triton X‐100/PBS for 15 min. 1% BSA (New England Biolabs, Ipswich, USA) was used for blocking. .. After 10 min of blocking, the cells were incubated with the primary antibodies anti‐TFF2 (Protein tech, Rosemont, IL, USA, 13681‐1‐AP, 1:100 dilution) for 1 h at 25 ̊C room temperature. .. Cells were washed three times with PBS and further incu‐ bated them with anti‐rabbit horseradish‐conjugated secondary antibodies (Dako, Glostrup, Denmark, 1:1,000 dilution) for 30 min at room temperature.

    Formalin-fixed Paraffin-Embedded:

    Article Title: A novel telomere biology disease‐associated gastritis identified through a whole exome sequencing‐driven approach
    Article Snippet: Anti-Pepsinogen 1 antibody (1:400) was applied on tissue sections for one-hour incubation at room temperature and the antigen-antibody binding was detected with Bond Polymer Refine Detection (Leica Biosystems, DS9800). .. Tissue sections were briefly immersed in hematoxylin for counterstaining and were covered with cover glasses. c. Protocol for anti-TFF2 (ProteinTech Group, catalog# 13681-1-AP, rabbit polyclonal, 400 μg/ml) on human FFPE GI tissues: The slide was stained using Leica Bond RX automated stainer. .. After deparaffinization and rehydration, tissue section was heat treated for 20 minutes with antigen retrieval solution (Leica Biosystems, AR9961).

    Immunohistochemistry:

    Article Title: YAP targetome reveals activation of SPEM in gastric pre-neoplastic progression and regeneration.
    Article Snippet: .. Rabbit polyclonal anti-YAP (Cell signaling, 14074, 1:200), anti-TFF2 (Proteintech, 13681-1-AP, 1:1000) and anti-HE4 (Proteintech, 14406-1-AP, 1:200) antibodies were used for immunohistochemistry. ..

    Blocking Assay:

    Article Title: The expression of trefoil factor family member 2 in increased at an acidic pH.
    Article Snippet: The cells were permea‐ bilized with 0.2% Triton X‐100/PBS for 15 min. 1% BSA (New England Biolabs, Ipswich, USA) was used for blocking. .. After 10 min of blocking, the cells were incubated with the primary antibodies anti‐TFF2 (Protein tech, Rosemont, IL, USA, 13681‐1‐AP, 1:100 dilution) for 1 h at 25 ̊C room temperature. .. Cells were washed three times with PBS and further incu‐ bated them with anti‐rabbit horseradish‐conjugated secondary antibodies (Dako, Glostrup, Denmark, 1:1,000 dilution) for 30 min at room temperature.

    Article Title: Systematic identification of cell-fate regulatory programs using a single-cell atlas of mouse development.
    Article Snippet: .. Primary antibodies (anti-ESAM (1:50; Thermo Fisher Scientific, catalog no. MA5-24072), anti-Myl9 (1:400; Abcam, catalog no. ab187152), anti-Scgb1a1 (1:50, R&D, catalog no. MAB4218-SP), anti-tff2 (1:200; ProteinTech, catalog no. 13681-1-AP) and anti-AFP (1:200, Affinity, catalog no. AF5134)) diluted in blocking solution were added to cover the sections. .. Primary antibodies (anti-ESAM (1:50; Thermo Fisher Scientific, catalog no. MA5-24072), anti-Myl9 (1:400; Abcam, catalog no. ab187152), anti-Scgb1a1 (1:50, R&D, catalog no. MAB4218-SP), anti-tff2 (1:200; ProteinTech, catalog no. 13681-1-AP) and anti-AFP (1:200, Affinity, catalog no. AF5134)) diluted in blocking solution were added to cover the sections.



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    Scavenging saliva fluid MIF and <t>TFF2</t> ameliorates effects of saliva fluid on development of DSS-induced colitis. ( A and B ) Standard curves ( left ) and the level of MIF ( A ) and TFF2 ( B ) in saliva from control ( black ) and DSS-treated mice ( red ) and saliva treated with the respective antibodies ( green ). ( C and E ) WT ( C ) and Aqp5 -/- mice ( E ) were fed with water ( black ) or 3% DSS ( blue, green and red ) for 6 days and allowed to recover for 6 days. The mice were gavaged with the salivary fluid treated with anti-MIF ( green ) <t>and</t> <t>anti-TFF2</t> antibodies ( red ) on days 1, 3, and 6, as indicated. Mice were 18 to 22 weeks old, and the numbers in parenthesis indicate the number of mice in each group. The P values are relative to mice fed with DSS. The results with DSS shown in dotted lines were taken from and are shown to better illustrate the effect of MIF and TFF2 depletion. ( D and F ) Time course of development of disease activity of the mice in ( C and E ), respectively. ( G ) Intestinal length measured on day 8. ( H and I ) Analysis of cell proliferation ( left ) and goblet cell density ( right ). ( J and K ) Intestinal ( J ) and serum ( K ) MPO levels. ( L ) Intestinal permeability expressed as fold change measured on day 8. ( M and N ) Analysis of ZO1 ( M ) and Occludin ( N ) measured on day 8.
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    Stem cell characteristics of CD44v9-positive cells induced by H pylori G27 strains (CagA-positive H pylori ) infection in the presence of butyrate (But). Immunofluorescence analysis of colocalization between CD44v9 and stem cell markers (LGR5, KLF5, and SALL4) in mucosoids derived from the gastric corpus and antrum of mice infected with H pylori G27 strains (CagA-positive H pylori ) or H pylori G27 cag PAI-deletion mutant strains (Δ cag PAI) in the presence of But. Colocalization of CD44v9 and the SPEM marker <t>TFF2</t> was also assessed. Scale bar = 50 μm.
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    Image Search Results


    Scavenging saliva fluid MIF and TFF2 ameliorates effects of saliva fluid on development of DSS-induced colitis. ( A and B ) Standard curves ( left ) and the level of MIF ( A ) and TFF2 ( B ) in saliva from control ( black ) and DSS-treated mice ( red ) and saliva treated with the respective antibodies ( green ). ( C and E ) WT ( C ) and Aqp5 -/- mice ( E ) were fed with water ( black ) or 3% DSS ( blue, green and red ) for 6 days and allowed to recover for 6 days. The mice were gavaged with the salivary fluid treated with anti-MIF ( green ) and anti-TFF2 antibodies ( red ) on days 1, 3, and 6, as indicated. Mice were 18 to 22 weeks old, and the numbers in parenthesis indicate the number of mice in each group. The P values are relative to mice fed with DSS. The results with DSS shown in dotted lines were taken from and are shown to better illustrate the effect of MIF and TFF2 depletion. ( D and F ) Time course of development of disease activity of the mice in ( C and E ), respectively. ( G ) Intestinal length measured on day 8. ( H and I ) Analysis of cell proliferation ( left ) and goblet cell density ( right ). ( J and K ) Intestinal ( J ) and serum ( K ) MPO levels. ( L ) Intestinal permeability expressed as fold change measured on day 8. ( M and N ) Analysis of ZO1 ( M ) and Occludin ( N ) measured on day 8.

    Journal: Cellular and Molecular Gastroenterology and Hepatology

    Article Title: Role of Innate Oral Immunity and the Salivary Fluid in Inflammatory Bowel Disease

    doi: 10.1016/j.jcmgh.2025.101713

    Figure Lengend Snippet: Scavenging saliva fluid MIF and TFF2 ameliorates effects of saliva fluid on development of DSS-induced colitis. ( A and B ) Standard curves ( left ) and the level of MIF ( A ) and TFF2 ( B ) in saliva from control ( black ) and DSS-treated mice ( red ) and saliva treated with the respective antibodies ( green ). ( C and E ) WT ( C ) and Aqp5 -/- mice ( E ) were fed with water ( black ) or 3% DSS ( blue, green and red ) for 6 days and allowed to recover for 6 days. The mice were gavaged with the salivary fluid treated with anti-MIF ( green ) and anti-TFF2 antibodies ( red ) on days 1, 3, and 6, as indicated. Mice were 18 to 22 weeks old, and the numbers in parenthesis indicate the number of mice in each group. The P values are relative to mice fed with DSS. The results with DSS shown in dotted lines were taken from and are shown to better illustrate the effect of MIF and TFF2 depletion. ( D and F ) Time course of development of disease activity of the mice in ( C and E ), respectively. ( G ) Intestinal length measured on day 8. ( H and I ) Analysis of cell proliferation ( left ) and goblet cell density ( right ). ( J and K ) Intestinal ( J ) and serum ( K ) MPO levels. ( L ) Intestinal permeability expressed as fold change measured on day 8. ( M and N ) Analysis of ZO1 ( M ) and Occludin ( N ) measured on day 8.

    Article Snippet: The depletion of MIF or TFF2 protein from the saliva fluid was by incubation of the fluid for overnight at 4°C with anti-MIF (Cell Signaling, Cat no.88186S) or anti-TFF2 (ProteinTech, Cat no. 13681-1-AP) neutralizing antibodies at a dilution of 5 μg/mL for both peptides.

    Techniques: Control, Activity Assay, Permeability

    Stem cell characteristics of CD44v9-positive cells induced by H pylori G27 strains (CagA-positive H pylori ) infection in the presence of butyrate (But). Immunofluorescence analysis of colocalization between CD44v9 and stem cell markers (LGR5, KLF5, and SALL4) in mucosoids derived from the gastric corpus and antrum of mice infected with H pylori G27 strains (CagA-positive H pylori ) or H pylori G27 cag PAI-deletion mutant strains (Δ cag PAI) in the presence of But. Colocalization of CD44v9 and the SPEM marker TFF2 was also assessed. Scale bar = 50 μm.

    Journal: Gastro Hep Advances

    Article Title: Helicobacter pylori Exploit Short-Chain Fatty Acids-Induced CAPZA1 Overexpression to Emerge CD44v9-Positive Stemness

    doi: 10.1016/j.gastha.2025.100860

    Figure Lengend Snippet: Stem cell characteristics of CD44v9-positive cells induced by H pylori G27 strains (CagA-positive H pylori ) infection in the presence of butyrate (But). Immunofluorescence analysis of colocalization between CD44v9 and stem cell markers (LGR5, KLF5, and SALL4) in mucosoids derived from the gastric corpus and antrum of mice infected with H pylori G27 strains (CagA-positive H pylori ) or H pylori G27 cag PAI-deletion mutant strains (Δ cag PAI) in the presence of But. Colocalization of CD44v9 and the SPEM marker TFF2 was also assessed. Scale bar = 50 μm.

    Article Snippet: The following antibodies were used for fluorescence immunocytochemistry: anti-CAPZA1 (OTI2G4) (Thermo Fisher Scientific, cat# MA5-25093; 1:500 or Abcam, Cambridge, UK, cat# ab166892; 1:500), anti-CD44v9 (Cosmo Bio, Tokyo, Japan, cat# CAC-LKG-M001; 1:500), anti-CagA (Austral Biologicals, cat# HPM-5001–5; 1:500), anti-LGR5 (Atlas antibodies, Stockholm, Sweden, cat# HPA012530; 1:200), anti-KLF5 (GeneTex, Irvine, CA, cat#GTX103289; 1:200), anti-SALL4 (Abcam, cat# ab29112; 1:200), and anti-trefoil factor 2 (TFF2) (Proteintech, Wuhan, China, cat# 13681-1-AP; 1:200).

    Techniques: Infection, Immunofluorescence, Derivative Assay, Mutagenesis, Marker